| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Diguey, Velina (10)
|
MAUN |
39.20 |
37.89 |
-1.31 |
| 1 |
Olmstead, Elise (10)
|
MALYM |
44.71 |
42.09 |
-2.62 |
| 1 |
Zelin, Brooke A (10)
|
MAPVAC |
|
44.06 |
- |
| 2 |
Reasons, Kayla J (9)
|
MARAD |
39.35 |
38.99 |
-0.36 |
| 2 |
Glynn, Delaney C (10)
|
MANSC |
43.96 |
43.25 |
-0.71 |
| 2 |
Abruzzo, Gabrielle R (9)
|
MAPWAC |
|
44.13 |
- |
| 3 |
Gallagher, Rachel J (10)
|
MAUN |
40.73 |
41.00 |
0.27 |
| 3 |
Foppa, Ashley (9)
|
MAUN |
47.95 |
44.54 |
-3.41 |
| 3 |
Maurya, Anshi (10)
|
MAUMAC |
45.56 |
45.83 |
0.27 |
| 4 |
Bradwell, Gwen Z (10)
|
MABSC |
49.27 |
45.53 |
-3.74 |
| 4 |
Hendrickson, Liliana J (10)
|
MAEAAC |
45.73 |
46.01 |
0.28 |
| 5 |
Enakhimion, Emiola A (10)
|
MAEAAC |
47.97 |
45.92 |
-2.05 |
| 5 |
Short, Sophia N (9)
|
MANSC |
47.63 |
47.40 |
-0.23 |
| 6 |
Doig, Melissa (9)
|
MABRY |
48.52 |
46.48 |
-2.04 |
| 7 |
Archer, Elizabeth M (10)
|
MAPWAC |
51.14 |
46.60 |
-4.54 |
| 8 |
Flicker, Maya R (9)
|
MACYL |
54.25 |
48.53 |
-5.72 |
| 9 |
Hickey, Lillian G (9)
|
MATRI |
49.25 |
49.30 |
0.05 |
| 10 |
Owens, Ava L (9)
|
MACYL |
51.99 |
49.52 |
-2.47 |
| 11 |
Vassallo, Margherita (10)
|
MARAD |
|
50.01 |
- |
| 12 |
Barbash, Elizabeth P (10)
|
MAUN |
50.97 |
50.16 |
-0.81 |
| 13 |
Nikolic, Daria M (9)
|
MABSC |
52.65 |
50.66 |
-1.99 |
| 14 |
Calvert, Chloe V (10)
|
MATRI |
1:05.43 |
51.14 |
-14.29 |
| 15 |
Coombs, Ava (9)
|
MAPWAC |
|
53.53 |
- |
| 16 |
Meyers, Sophia M (10)
|
MANSC |
55.41 |
54.69 |
-0.72 |
| 17 |
Albani, Emma M (10)
|
MAEAAC |
57.12 |
55.07 |
-2.05 |
| 18 |
Baggini, Julia R (10)
|
MAEAAC |
|
57.33 |
- |
| 19 |
Bush, Emma J (10)
|
MATRI |
1:08.69 |
1:02.11 |
-6.58 |
| 20 |
Grandizio, Madison M (10)
|
MATRI |
|
1:06.34 |
- |
|
Dimos, Olga (10)
|
MAEAAC |
|
47.56 |
- |
|
Johnston, Betty A (9)
|
MAEAAC |
|
54.35 |
- |
|
Gatta, Georgia G (9)
|
MAEAAC |
|
58.96 |
- |
|
Oswald, Delaney R (10)
|
MARAD |
46.28 |
NS |
- |